A quantitative luminescence assay for nonradioactive nucleic acid probes.
نویسندگان
چکیده
منابع مشابه
Immunological capture of nucleic acid hybrids and application to nonradioactive DNA probe assay.
Antibodies specific for DNA:RNA hybrids were coated onto polystyrene test tubes and applied to hybridization assays involving DNA and RNA. Synthetic DNA probes complementary to 16S rRNA of Campylobacter were labeled with biotin and hybridized to ribosomal RNA directly in lysates of bacterial cells. After hybridization, DNA:RNA hybrids were captured with immobilized anti-DNA:RNA antibody, and th...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1996
ISSN: 0022-1554,1551-5044
DOI: 10.1177/44.6.8666750